Sunday, June 10, 2018

6:00 – 7:00 PM Registration
7:00 – 9:00 PM Welcome Reception Dinner
9:00 – 10:00 PM Keynote Lectures
Chairperson Mavis Agbandje-McKenna
KL-1: 9:00 – 9:30 PM Peter Tijssen
The Extent of Diversity in Parvovirus Expression is Paramount with Densoviruses
KL-2: 9:30 – 10:00 PM Maria Söderlund-Venermo
Clinical Impact and Epidemiology of Emerging Human Parvoviruses

Monday, June 11, 2018

7:00 – 8:30 AM Breakfast
8:40 – 10:00 AM Session 1: Novel Parvoviruses
Chairpersons Mylène Ogliastro and Mario Mietzsch
O-1: 8:40 – 9:00 AM Robert McKenna, Mario Mietzsch, Mengxiao Luo, Shweta Kailasan, Maria Ilyas, Paul Chipman, Duncan Sousa, Maria Söderlund-Venermo, and Mavis Agbandje-McKenna
Structural Characterization of Emerging Human Protoparvoviruses
O-2: 9:00 – 9:20 AM Di Pasquale G, Chandler RJ, Choi EY, Hubbard B, Afione S, Khalaj M, Zheng C, Grewe B, Kaler SG, Venditti CP, and Chiorini JA
Characterization of AAV44.9, a Member of the New AAV Clade, with a Tropism Capable of Treating a Broad Spectrum of Genetic Disease
O-3: 9:20 – 9:40 AM Judit J Penzes, Hanh T Pham, Mavis Agbandje-McKenna, and Peter Tijssen
A Novel Densovirus of the Prawn Peneaus monodon of a New Lineage within Subfamily Densovirinae Harbors a Unique Genome Organization, Transcription Pattern, and Possible Cellular Trafficking Routes
O-4: 9:40 – 10:00 AM Carlos Gallego, Nooshin Bayat, David Abia, and José M. Almendral
Endogenous Parvovirus Sequences in Animal Genomes: A Critical Assessment
10:00 – 10:40 AM Tea/Coffee Break
10:50 – 12:20 PM Session 2:  Viral Entry and Trafficking I
Chairpersons Rob McKenna and Colin Parrish
O-5: 10:50 – 11:00 AM Nancy L. Meyer, Guiqing Hu, Qing Xie, Omar Davulcu, Yoshi Ishakawa, Drew Gingerich, Craig Yoshioka, Larry David, Alex Noble, Wei Zou, Fang Cheng, Safder Ganaie, Xuefeng Deng, Sirika Pillay, Jan E. Carrette, Jianming Qiu, Scott M. Stagg, and Michael S. Chapman
Interactions of AAV-2 with its Cellular Receptor (AAVR), Visualized by Cryo-Electron Microscopy
O-6: 11:00 – 11:20 AM Jan Bieri and Carlos Ros
Globoside Receptor of Human Parvovirus B19 is Dispensable for Virus Binding, Uptake and Nuclear Targeting but is Required for the Infection
O-7: 11:20 – 11:40 AM Justin Kurian, Bridget Lins, Antonette Bennett, Robert McKenna, and Mavis Agbandje-McKenna
Endo/Lysosomal pH-Induced Structural Changes are Conserved in Adeno-Associated Virus Capsids
O-8: 11:40 – 12:00 PM Antonette Bennett, Julie Tordo, Katie Moss, Joshua Hull, Enswert Barnes, Nelly Jolinon, Michael Linden, Els Henckaerts, and Mavis Agbandje-McKenna
Comparative Studies of AAV2 True Type and AAV2 Wild Type
O-9: 12:00 – 12:20 PM Yuan Lu, Jinsong Wei, Gai Ran, Chenghui Yu, Lingling Zhao, Bing Zhao, and Chen Ling
High-Efficiency Genetic Engineering of Stem Cell Organoids using Recombinant Adeno-Associated Virus
12:30 – 2:20 PM Lunch and Poster Session I
2:30 – 4:10 PM Session 3: Viral Entry and Trafficking II
Chairpersons Carlos Ros and Giovanni Di Pasquale
O-10: 2:30 – 2:50 PM Mario Mietzsch, Mengxiao Luo, Shweta Kailasan, Maria Ilyas, Paul Chipman, Chen Xu, Kalle Kantola, Duncan Sous, Robert McKenna, Maria Söderlund-Venermo, and Mavis Agbandje-McKenna
The Capsid Structure of Human Bocaviruses
O-11: 2:50 – 3:10 PM Oliver Caliaro, Nico Ruprecht, and Carlos Ros
Capsid Structural Transitions Associated with Uncoating and Nuclear Entry of Human Parvovirus B19
O-12: 3:10 – 3:30 PM Amanda M. Dudek, Eric M. Zinn, Sirika Pillay, Andreas S. Puschnik, Claude M. Nagamine, Fang Cheng, Jianming Qiu, Jan E. Carette, and Luk H. Vandenberghe
Identification and Characterization of an Alternate AAVR Independent AAV Entry Mechanism Using a Genome-Wide CRISPR/Cas9 Knock-Out Screen
O-13: 3:30 – 3:50 PM Eric D. Kelsic, Pierce J. Ogden, Sam Sinai, and George M. Church
Systematic Functional Characterization of the AAV Capsid Fitness Landscape
O-14: 3:50 – 4:10 PM Vesa Aho, Elina Mäntylä, Axel Ekman, Taro Ohkawa, Teemu Ihalainen, Salla Mattola, Satu Hakanen, Michael Kann, Loy Volkman, Matthew D. Welch, Carolyn A. Larabell, and Maija Vihinen-Ranta
Intranuclear Movement of Viruses through the Chromatin Network
4:10 – 4:30 PM Tea/Coffee Break
4:30 – 5:50 PM Session 4:  DNA Replication
Chairpersons Chen Ling and Victoria Madigan
O-15: 4:30 – 4:50 PM N.C. Horton, J.L. Sanchez, L.E. Basantes, and C. Barahona
Cooperative Sequence Specific Assembly of Human Parvovirus B19 NS1 Nuclease Domain at Promoter and Origin Sequences
O-16: 4:50 – 5:10 PM Peng Xu, Safder S. Ganaie, Zekun Wang, Nancy Horton, John Tavis, and Jianming Qiu
Development of a Novel Fluorophore-Based Drug Screening Assay by Targeting Endonuclease Activity of Parvovirus B19 NS1
O-17: 5:10 – 5:30 PM Sarah Smith-Moore, Stuart J.D. Neil, Cornel Fraefel, R. Michael Linden, Mathieu Bollen, Helen M. Rowe, and Els Henckaerts
Adeno-Associated Virus Rep Proteins Antagonize Phosphatase PP1 to Counteract KAP1 Repression of the Latent Viral Genome
O-18: 5:30 – 5:50 PM Vishaka Santosh and Carlos R. Escalante
Cryo-EM Structure of Rep68-ssDNA complex
5:50 – 7:00 PM Poster Session II
7:00 PM Dinner

Tuesday, June 12, 2018

7:00 – 8:30 AM Breakfast
8:40 – 10:00 AM Session 5: Gene Expression
Chairpersons Jianming Qiu and Kinjal Majumder
O-19: 8:40 – 9:00 AM Igor Etingov, Kinjal Majumder, and David J. Pintel
Checkpoint Kinase 1 (Chk1) Plays an Important Role in MVM Infection
O-20: 9:00 – 9:20 AM Victoria J. Madigan, Julianne Yuziuk, L. Victor Tse, Anna Chiarella, Tyne Tyson, Nate Hathaway, and Aravind Asokan
RNF121 is a Key Transcriptional Regulator of AAV Genome Expression
O-21: 9:20 – 9:40 AM Chenghui Yu, Gai Ran, Jingwei Chen, Jian Hou, and Chen Ling
Capsid Protein of Various AAV Serotypes are Involved in the Transcription of Viral Genome
O-22: 9:40 – 10:00 AM Himanshu K. Rambhai and Arun Srivastava
Role of Essential Metal Ions in AAV Vector Biology
10:00 – 10:40 AM Tea/Coffee Break
10:50 -12:20 PM Session 6: Virus-Host Interactions
Chairpersons Maija Vihinen-Ranta and Carlos Escalante
O-23: 10:50 – 11:00 AM Colin Parrish, Heather Callaway, Susan Hafenstein, and Hyunwook Lee
Variation in parvovirus capsids controls the specific structural interactions with transferrin receptors or with antibodies, and effects host-specific infection
O-24: 11:00 – 11:20 AM Hyunwook Lee, Heather Callaway, Colin R. Parrish, and Susan Hafenstein
The Structure of the Transferrin Receptor:Canine Parvovirus Complex Reveals How the Interactions Control Viral Host Range
O-25: 11:20 – 11:40 AM Chingakham Ranjit Singh, Sarah Gillaspie, Mackenzie Thornton, Maureen Schick, Giovanni Di Pasquale, Sandra Afione, John A. Chiorini, and Katsura Asano
Translational Control of Adeno-Associated Virus 2 by eIF5-Mimic Protein
O-26: 11:40 – 12:00 PM Kinjal Majumder, Juexin Wang, Maria Boftsi, Jordan E. Rede, Matthew S. Fuller, Trupti Joshi, and David J. Pintel
The Parvovirus Minute Virus of Mice Localizes to Sites of Cellular DNA Damage to Establish and Amplify its Lytic Infection
O-27: 12:00 – 12:20 PM L. Pigeyre, M. Ravallec, L. Gasmi, N. Nègre, C. Clouet, Y. Guerardel, D. Cot, T. Dupressoir, A.-S. Gosselin-Grenet, and M. Ogliastro
Densovirus Oral Infection Targets and Disrupts the Peritrophic Matrix of the Lepidopteran Pest Spodoptera frugiperda
12:30 – 2:20 PM Lunch and Poster Session III
2:30 -3:50 PM Session 7: Capsid Assembly and Packaging
Chairpersons Aravind Asokan and Nathalie Clément
O-28: 2:30 – 2:50 PM Qian Yu, Peng Lu, Yali Xing, and Qin Yao
The Transcription Strategy of Bombyx mori Bidensovirus and a Characterization of the Viral Structural Proteins
O-29: 2:50 – 3:10 PM Qizhao Wang, Junping Zhang, Ping Guo, Nianli Sang, Jenni Firrman, George Wang, Yong Diao, and Weidong  Xiao
Systemic Evaluation of Host and Viral Elements Affecting AAV Encapsidation
O-30: 3:10 – 3:30 PM Longping V. Tse, Sven Moller-Tank, Rita M. Meganck, and Aravind Asokan
Mapping and Engineering Functional Domains of the Assembly Activating Protein of Adeno-Associated Viruses
O-31: 3:30 – 3:50 PM Anna  C.  Maurer, Ana Karla Cepeda Diaz, and Luk H. Vandenberghe
The Assembly Activating Protein Pleiotropically Supports Adeno-Associated Virus Production
3:55 – 4:30 PM Tea/Coffee Break
4:30 – 5:50 PM Session 8:  Viral Epidemiology and Evolution
Chairpersons Maria Söderlund-Venermo and Anne-sophie Gosselin-Grenet
O-32: 4:30 – 4:50 PM Doriane Mutuel, Laetitia  Pigeyre, Ki-Woong Nam, Anne-Sophie Gosselin Grenet, Rémy Froissart, and Mylène Ogliastro
Densovirus Experimental Evolution in Insecta: The Appearance Can be Deceptive
O-33: 4:50 – 5:10 PM L Patrick Havlik, L Victor Tse, Daniel Oh, Kelli Klinc, Mavis Abgandje-McKenna, and Aravind Asokan
Dissecting AAV Structure-Function Correlates Through Directed Evolution
O-34: 5:10 – 5:30 PM Tarja Sironen, Anna-Maria Moisander-Jylhä, Jenni Virtanen, Kirsi Aaltonen, and Olli Vapalahti
Parvoviruses of Mink and Fox – Molecular Epidemiology and Prevention
O-35: 5:30 – 5:50 PM Luk H. Vandenberghe
Statistical, Evolutionary, and Multiplex Methods to Map Phenotypic Variation onto Rigid Viral Structures
6:30 PM Cruise Dinner

Wednesday, June 13, 2018

7:00 – 8:30 AM Breakfast
8:40 – 10:00 AM Session 9: Clinical Parvovirology
Chairpersons David Pintel and Victor Tse
O-36: 8:40 – 9:00 AM Ivailo Mihaylov, Matthew Marr, Serena Lian, Anthony D’Abramo, Jr., Lei Li, Nikea Pittman, Mavis  Agbandje-McKenna, Susan F. Cotmore, and Peter Tattersall
Optimizing the Transgene Capacity of Rodent Protoparvovirus Vectors Targeting Murine Melanoma Cells
O-37: 9:00 – 9:20 AM Tania Calvo, Esther Grueso, Cristina Sánchez-Martínez, and José M. Almendral
Engineering MVM Capsid Loci with VEGF-R Binding Peptides: Structural, Immune, and Re-Targeting Implications
O-38: 9:20 – 9:40 AM Altinli M., Atyame C.N., Gunay F., Alten B., Bonneau M., Soms J., Ogliastro M., Weill M., Gosselin-Grenet A.S., and Sicard M.
Prevalence and Transmission of Culex pipiens Densovirus
O-39: 9:40 – 10:00 PM Oleksandr Kondratov, Damien Marsic, Shreyasi Choudhury, Sanford L. Boye, Shannon E. Boye, and Sergei Zolotukhin
Application of Next-Generation Sequencing in Viral Library Screening
10:05 – 10:40 AM Tea/Coffee Break
10:50 – 12:20 PM Session 10: Gene Therapy I
Chairpersons Jay Chiorini and Antonette Bennett
O-40: 10:50 – 11:00 AM Julie Tordo, Claire O’Leary, André S.L.M. Antunes, Nuria Palomar, Patrick Aldrin-Kirk, Mark Basche, Antonette Bennett, Zelpha D’Souza, Hélène Gleitz, Annie Godwin, Rebecca Holley, Helen Parker, Ai Yin Liao, Paul Rouse, Amir Saam Youshani, Larbi Dridi, Carla Martins, Thierry Levade, Kevin Stacey, Daniel M. Davis, Adam Dyer, Nathalie Clément, Tomas Björklund, Robin R. Ali, Mavis Agbandje-McKenna, Ahad A. Rahim, Alexey Pshezhetsky, Simon N.  Waddington, R. Michael Linden, Brian W. Bigger, and Els Henckaerts
A Novel Adeno-Associated Virus Capsid with Enhanced Neurotropism Corrects a Lysosomal Transmembrane Enzyme Deficiency
O-41: 11:00 – 11:20 AM Cristhian J. Ildefonso, Virginia White, Qiuhong Li, and Alfred S. Lewin
AAV Delivery of Genes for Secreted Cell Penetrating Proteins to Treat Retinal Inflammation
O-42: 11:20 – 11:40 AM Jason Wright, Laura Smith, Laura Adamson-Small, Seemin  Ahmed, Nancy Avila, Brenda Burnham, Saswati Chatterjee, Serena Dollive, Jeff Ellsworth, Deiby Faulkner, Omar Francone, Monicah Kivaa, Dave Knowlton, Jaime Prout, Hill Rubin, Meghan Scarpitti, Danielle Sookiasian, Arnold Sengooba, Thia St. Martin, and Albert Seymour
Nuclease-Free Genome Editing by AAVHSC Vectors Leads to in vivo Genome Correction and Amelioration of Disease Phenotype in a Mouse Model of Phenylketonuria (PKU)
O-43: 11:40 – 12:00 PM Lakmini Wasala, Yongping Yue, Junling Zhao, Yi Lai, and Dongsheng Duan
Implication of Dystrophin Hinge Regions in Micro-Dystrophin Gene Replacement Therapy for Duchenne Muscular Dystrophy
O-44: 12:00 – 12:20 PM Sanford L. Boye, James P. Peterson, Michael Lukason, Diego Fajardo, Hangning Zhang, Catherine O’Riordan, Rena Beck, Carol Sherako, Samuel G. Jacobson, Alison McVie-Wylie, and Shannon E. Boye
Pre-Clinical Development of an AAV Based Gene Therapy for the Treatment of Retinal Disease Due to Recessive Mutations in GUCY2D
12:30 – 2:30 PM Lunch and Poster Session III
2:30 – 3:50 PM Session 11:  Gene Therapy II
Chairpersons Els Henckaerts and Sanford Boye
O-45: 2:30 – 2:50 PM Alexandra S. Agostinho, Mario Mietzsch, Luca Zangrandi, Iwona Kmiec, Anna Mutti, Larissa Kraus, Pawel Fidzinski, Ulf C. Schneider, Martin Holtkamp, Christoph Schwarzer, and Regine Heilbronn
Neuropeptide-Based Gene Therapy for Treatment of Focal Epilepsy
O-46: 2:50 – 3:10 PM A. Gary Todd, Lauren A. Vaught, Lauren Duncanson, David D. Fuller, Barry J. Byrne, and Darin J. Falk
Gene Therapy Improves Lifespan and Cardiorespiratory Function in a Rat Model of Pompe Disease
O-47: 3:10 – 3:30 PM Zekun Wang, John F. Engelhardt, Ziying Yan, and Jianming Qiu
Development of a Novel Recombinant Adeno-Associated Virus Production System Using Human Bocavirus 1 Helper Genes
O-48: 3:30 – 3:50 PM R. Jude Samulski and Xiao Xiao
Gene Therapy for DMD: From Bench to Bedside
3:50 – 4:30 PM Tea/Coffee Break
4:45 PM Group Picture
5:00 – 6:00 PM Keynote Lectures
Chairperson Arun Srivastava
KL-3: 5:00 – 5:30 PM Kenneth I. Berns
AAV ITR: Not at the End Yet
KL-4: 5:30 – 6:00 PM Nicholas Muzyczka
The History of 40 Years of AAV Gene Therapy
7:00 – ?? PM Gala Dinner and Awards Presentations

Posters

Monday, June 11, 2018 – Wednesday, June 13, 2018

P-1 Jing Cai, Xue Mi, and Yong Diao

Salvianolic Acid B Increases Recombinant Adeno-associated Virus Transduction through Redirecting Trafficking Pathways and Stabilizing Perinuclear Accumulations

P-2 Kinjal Majumder, Juexin Wang, Maria Boftsi, Jordan E. Rede, Matthew S. Fuller, Trupti Joshi, and David J. Pintel

The Parvovirus Minute Virus of Mice Localizes to Sites of Cellular DNA Damage that form Distinct Chromatin Domains to Establish and Amplify its Lytic Infection

P-3 Maria Boftsi, Kinjal Majumder, and David J. Pintel

Mutations in the CTCF Binding Sites on the MVM Genome Reduces Capsid Protein Expression

P-4 Yanming Dong, Olufemi O. Fasina, and David J. Pintel

The Minute Virus of Canines NP1 Protein Affects Multiple Aspects of Viral Gene Expression via its Role in Alternative RNA Processing

P-5 Mario Mietzsch, Victoria E. Fielding, Judit Penzes, Qian Yu, Paul Chipman, Robert McKenna, Giovanni DiPasquale, John Chiorini, Ya Li, Lin Yang, Sergei Zolotukhin, Peter Tijssen, and Mavis Agbandje-McKenna

Structure-Function Characterization of Non-Primate AAVs

P-6 Zekun Wang, Weiran Shen, Fang Cheng, Xuefeng Deng, John F. Engelhardt, Ziying Yan, and Jianming Qiu

Human Parvovirus Expresses a Long Noncoding RNA that is Essential to Virus Replication

P-7 Jenni Virtanen, Teemu Smura, Kirsi Aaltonen, Anna-Maria Moisander-Jylhä, Anna Knuuttila, Olli Vapalahtia, and Tarja Sironen

Co-Circulation of Highly Diverse Aleutian Mink Disease Virus Strains in Finland

P-8 Sereina O. Sutter, Kurt Tobler, Michael Seyffert, Elisabeth M. Schraner, Bernd Vogt, and Cornel Fraefel

IFI16 is a Restriction Factor of AAV2 Infection

P-9 Man Xu, Katarzyna Leskinen, Valerija Groma, Yilin Wang, Anna Lepistö, Klaus Hedman, Taina Sipponen, Päivi Saavalainen, and Maria Söderlund-Venermo

Occurrence, Activity and Impact of Human Parvovirus Persistence in Diseased and Healthy Gut

P-10 Mario Mietzsch, Jennifer C. Yu, Felix Bröcker, Jun Xie, Robert McKenna, Duncan Sousa, Peter H. Seeberger, Guangping Gao, Regine Heilbronn, and Mavis Agbandje-McKenna

Comparative Analysis of the Capsid Structures of AAV8, AAVrh.10, and AAVrh.39 and their Glycan Interactions

P-11 Nelly Jolinon, Antonette Bennett, Julie Tordo, Patrick Aldrin-Kirk, Adam Dyer, Tomas Björklund, R. Michael Linden, Mavis Agbandje-McKenna and Els Henckaerts

Functional Characterization of AAV-TT

P-12 Ariana Jose, Mario Mietzsch, Kennon Smith, Justin Kurian, Paul Chipman, Robert McKenna, John Chiorini, Mavis Agbandje-McKenna

Characterizing a Novel Antibody Epitope on the AAV5 Capsid at Atomic Resolution for the Creation of Host Antibody Resistant Recombinant Gene Delivery Vectors

P-13 Laura Smith, Hillard Rubin, Jacinthe Gingras, Albert Seymour, Pablo Morales, Elizabeth Chlipala, Michael O’Callaghan, and Jeff Ellsworth

Widespread Transduction of the Central Nervous System Following Systemic Delivery of AAVHSC7, AAVHSC15 and AAVHSC17 in Non-Human Primates

P-14 Joshua A Hull, Yong Zi Tan, Sriram Aiyer, Mario Mietzsch, Robert McKenna, Joshua Grieger, R. Jude Samulski, Timothy S. Baker, Dmitry Lyumkis, and Mavis Agbandje-McKenna

Structural Insights into the Role of the Conserved Leucine Located at the Base of the 5-Fold Pore in AAV Infectivity

P-15 Suriyasri Subramanian, Carol M. Bator, Alexander M. Makhov, James F. Conway, Carlos Ros, and Susan Hafenstein

Structural Analysis of Parvovirus B19 VP1 Unique Region using Cryo-Electron Microscopy

P-16 Shanan Emmanuel, Nikea Pittman, Judit Penzes, Susan Cotmore, Peter Tattersall, and Mavis Agbandje-McKenna

Rodent Protoparvoviruses: Identifying the Amino Acid Residues Involved in Oncotropism and Oncolysis

P-17 Jeff L. Ellsworth, Hillard Rubin, and Albert Seymour

Transduction of Photoreceptor and Pigmented Epithelial Cells Following a Single Subretinal Injection of AAVHSC17 in Minipigs

P-18 Potter M, Chaudhuri P, Byrne BJ, and Clément N

Scalable Manufacturing Platform for Highly Potent AAV Vectors Preparations

Thursday, June 14, 2018

7:00 – 8:30 AM Breakfast and Farewell